Microbiological analysis of drinking water quality of ananthanar channel of kanyakumari district, tamil nadu, india. Total coliforms and escherichia coli in water by membrane. It must be appreciated that all that bacteriological analysis can prove is that, at the time of examination, contamination or bacteria indicative of faecal pollution, could or could not be demonstrated in a given sample of water using specified culture methods. Bacteriological analysis of drinking water pages with reference to book, from 92 to 96 zumra sami public health division, national institute of health, islamabad. In monitoring for attainment of the recreational use the appropriate indicator must be determined before sample collection. Bacteriological analysis of water by multiple tube method. Get a printable copy pdf file of the complete article 150k, or click on a page image below to browse page by page. Chapter 6 through chapter 26 cover injurious or toxic substances of domestic, agricultural, and industrial sources. Bacteriological analysis enclose form when sending sample to lab rev. This video tutorial demonstrates bacteriological analysis of water by multiple tube method. Use only the laboratorysterilized bottle provided with sodium thiosulfate additive.
Pathogen indicators coliform streptococcus enterococcus 3. Laboratory staff for the collection of chemistry samples for drinking water analysis. Bacterial contamination of drinking water sources is the most common health risk. Bacteriological analysis of drinking water for private citizen, single household only collecting your water sample 1. The bacteriological examination of water is performed routinely by water utilities and many governmental agencies to ensure a safe supply of water for drinking, bathing, swimming and other domestic and industrial uses.
Fdas bacteriological analytical manual the bam is a collection of procedures preferred by analysts in u. In this method measured portions of a water sample are placed in testtubes containing a culture medium. Physicochemical and bacteriological analyses of water used for. In our study for microbiological water quality we followed next methodology. The research determines bacteriological quality of drinking water sources in serbo town, south west ethiopia. Sample collection procedure for bacteriological analysis of drinking water. Chapter 4 and chapter 5 discuss bacteriological and algal analysis. Water sampling and analysis world health organization. Humans consume and pollute large quantities of water. The d1974 form and the final report from the certified lab that analyzed your samples is due to be reported to dhec on the 10th of each month. Using a clean cloth outlet of the tap wipe to remove any dirt. Bacteriological analysis of drinking water sources article pdf available in african journal of microbiology research 518 september 2011 with 12,055 reads how we measure reads.
Please read all instructions before collecting water sample. Microbiological analysis of drinking water quality of. Physicochemical and bacteriological analyses of water. Results bacteriological analysis of water samples from four sources. It is the only procedure that can be used if water samples are very turbid or if semisolids such as sediments or sludges are to be analysed. Water contamination is one of the principal public health hazards associated with disasters, sinci contaminated water can set off an increase in gastroenteritis, diarrhea, and other waterborne diseases.
Food and drug administration laboratories for the detection in food and cosmetic. Disinfect the end of the faucet with an alcohol swab or dilute bleach solution 1 part household bleach to 10 parts water. Bio 3b laboratory bacteriological examination of water. Bacteriological water analysis is a method of analysing water to estimate the numbers of. Bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what sort of bacteria they are. Dnr drinking water program public water supply rev. Abstract water is one of the most important constituents of life support system.
Evaluation of bacteriological quality of surface, well. Bacteriological analysis of drinking water in zamfara. Bacteriological testing of water loughborough university. Quick guide to drinking water sample collection second. Data representation results obtained were represented using table and bar chart. The technique has been used for the analysis of drinkingwater for many years with satisfactory results. All coliforms will be detected at 37oc but only faecal coliforms at 44oc. Bacterial examination of water the bacteriological examination of water is performed routinely by water utilities and many governmental agencies to ensure a safe supply of water for drinking, bathing, swimming and other domestic and industrial uses.
Selecting an indicator for classified water bodies. The principal risk associated with water in small community supplies is that of infectious disease related to faecal contamination. Hence, as described in chapter 1, the microbiological examination of drinkingwater emphasizes assessment of the hygienic quality of the supply. Other uses include recreational, surface or marine water, bottled water, groundwater, well water, treatment plant effluents. It is a microbiological analytical procedure which uses samples of water and from these samples determines the concentration of bacteria. Turn on the tap for maximum flow and the water may run for two minutes. A good bacterial indicator of fecal pollution should fulfill the following criteria. Khan, abdul ghafoor pmrc central research centre, national institute of health, islamabad. Bacteriological analysis of well water sources in the. Abstract a total of 112 water samples in and around islamabad were assessed for bacteriological contamination.
Drinking water bacteriological analysis request form samples over 30 hours old and not in approved container are unsuitable for testing sampling procedure. Bacteriological analysis raw water pdf bromate analysis pdf bromide analysis pdf. The quality of water is of vital concern for mankind since it is directly linked with human welfare. Water quality monitoring world health organization. Once initially for community wells pollution usually expected to bacteriological testing only. Water sample for analysis should be collected in sterile bottle made up of neutral glass of capacity 200500 ml. Collection of water sample is important step in bacteriological examination of water. This note presents the background to the testing of water samples to determine whether diseasecausing bacteria, in. Pdf bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and, if needed, to find out what. Introduction water is one of the most important necessities to all forms of life on this planet and beyond.
Bacteriological analysis of well water sources in the bambui student residential area. The first, or presumptive test, is a screening test to sample water for the presence of coliform organisms. Bacteriological water analysis online biology notes. Bacteriological analysis of water worksheet lab number read by. Clean and safe drinking water is the basic need of human beings. Water contaminated with pathogenic species also has the normal inhabitants of the human intestine. Therefore adequate and safe water supply should be available to humans, plants, and animals in all parts of the world. However, now, the availability and quality of drinking water are questionable. Pdf bacteriological analysis of drinking water sources. Borehole water, physical, chemical, bacteriological analysis, enterobacteriaceae. This area for lab use only drinking water results sample bottles will often have plastic or paper seals on the cap, carefully remove this seal without touching the insides of the container.
The aerobic gram negative rods of the genera pseudomonas, alcalignes and flavobacterium as well as others are common in water. In this study a total of 22 drinking water samples, from the. One of the techniques is membrane filtration method. Bacteriological analysis of drinking water for private. Bacteriological analysis of water is performed to check the number of bacteria the water is contaminated with or simply it is a basic test for knowing about the water quality. Other approaches to sample collection may be acceptable or desirable under given conditions. Bacteriological quality of water samples in osogbo metropolis. A physicochemical and bacteriological analysis of borehole water samples from the wa municipality of the upper west region, ghana godfred etsey sebiawu, samuel asiedu fosu and sixtus bieranye bayaa martin saana dispensing technology department, wa polytechnic, wa. Domestic water is generally suppli ed to homes through private wells or public water com panies. Water samples, collected from different areas of delhi and ncr, have been analyzed for most probable number mpn test, as well as checked. Such contamination may take place at the source, at the. The total viable counts for all water samples were quite high ranging from 6. Pdf bacteriological quality analysis of tap water of. Bacteriological analyses were carried out on ananthanar channel water of kanyakumari district, tamil.
Where we are today still using analytical microbiological methods developed over a century ago in the uk, good quality water is the expected norm more than 99. The term faecal coliform has been used in water microbiology to denote coliform. Results of the bacteriological analysis of the water sample are presented in table 2. Bacteriological analysis of water sampling precautions. Bacteriological quality analysis of tap water of karachi, pakistan. Analysis of results was done using microsoft excel package to explore and deduce the standard deviation and mean of the results as shown below. Full text full text is available as a scanned copy of the original print version. Chain of custody drinking water bacteriological analysis.
Bacteriological analysis of drinking water sources. The bacterial examination of water has been standardized into three tests. Sampling location identification code geographical location university female. Water is one of the most precious commodities of life and society. The user is urged to check with the laboratory performing the analysis to. The study was performed during the warm, rainy season, when prevalence of diarrhoea linked to bacterial contamination of water supply is usually at its highest level in tropical countries. The first of these is called the multiple fermentation tube or most probable number technique. Water of good drinking quality is of basic importance to human physiology as well as indispensable to mans con tinued existence 1. Many of these bacteria are capable of growing on a wide variety of single carbon sources. This document is intended as a refresher for those already trained in sample collection. If sample collection process is not appropriate, it gives false result and raises question about validity and liability of the test. The complete analysis consists of series of test to identify the bacteria. Enumeration methods membrane filter multiple tube fermentation 3. It is regularly tested for in coastal sea water samples, as well as runoff water.
Bacteriological quality of different water samples are shown in figure 1. African journal of clinical and experimental microbiology. Bacteriological analysis of water using the pour plate method, total heterotrophic bacteria in the water samples were obtained dhawale and lamaster, 2003. The examination of water supplies that have been disinfected with. Comparative bacteriological analysis of stored borehole.
The procedure followed is fundamental to bacteriological analyses and the test is used in many. System information to be completed by department of natural resourcessampler. Remove any screens, filters or aerator devices from your faucet and allow the water to run for several minutes. A study of the bacteriological quality of bottled and tap. Suite 716 san diego, ca 92110 patricia mcvay, md, chief lab director signature date remarks approved revised signature date remarks.